DNA Data File for DNA of HIV

LOCUS       HIVPRBAC      582 bp ds-DNA             SYN       15-SEP-1990

DEFINITION  Synthetic HIV type-I protease (syn BAC) gene, complete cds.

ACCESSION   M36450

KEYWORDS    HIV type-1 protease; HIV-PR gene.

SOURCE      Synthetic oligonucleotide DNA, clone pET3AM.

  ORGANISM  Artificial gene

            Artificial sequences; Genes.

REFERENCE   1  (bases 1 to 582)

  AUTHORS   Cheng,Y.-S.E., McGowan,M.H., Kettner,C.A., Schloss,J.V.,

            Erickson-Viitanen,S. and Yin,F.H.

  TITLE     High-level synthesis of recombinant HIV-1 protease and the recovery

            of active enzyme from inclusion bodies

  JOURNAL   Gene 87, 243-248 (1990)

  STANDARD  simple staff_entry

FEATURES             Location/Qualifiers

     CDS             7. .573

                     /note="HIV type-I protease (BAC)"

                     /codon_start=7

BASE COUNT      120 a    125 c    170 g    167 t

ORIGIN



 M36450  Length: 582  January 18, 1992  09:27  Type: N  Check: 1028  ..



       1  GAATTCATGG AAGATCTGGC TTTCCTGCAA GGTAAGGCTC GTGAGTTCTC 



      51  TTCTGAACAG ACTCGTGCTA ACTCTCCGAC TCGTCGTGAG CTGCAGGTTT 



     101  GGGGTCGTGA TAACAACTCT CCGTCTGAGG CTGGCGCCGA TCGTCAGGGT 



     151  ACCGTTTCTT TTAACTTTCC GCAGGTTACT CTGTGGCAGC GTCCACTGGT 



     201  TACTATTAAG ATTGGTGGTC AGCTGAAGGA AGCTCTGCTG GATACCGGTG 



     251  CTGATGATAC TGTACTGGAG GAGATGTCTC TCCCGGGCCG TTGGAAGCCG 



     301  AAGATGATCG GTGGTATTGG TGGTTTCATT AAGGTACGTC AGTATGATCA 



     351  GATTCTGATT GAGATCTGTG GCCATAAGGC GATCGGTACC GTTCTGGTGG 



     401  GCCCGACTCC GGTTAACATT ATCGGCCGTA ACCTGCTGAC TCAGATCGGT 



     451  TGTACTCTGA ACTTTCCGAT TTCTCCGATT GAGACTGTTC CGGTTAAGCT 



     501  GAAGCCGGGT ATGGATGGTC CGAAGGTTAA GCAGTGGCCA CTGACTGAGG 



     551  AGAAGATTAA GGCTCTGGTT TAATAGAAGC TT